| ABSTRACT |
Aqueous two-phase extraction is recognized as an effective, versatile and important emerging technique for the downstream processing of biomolecules. The advantages of alcohol/salt-based aqueous two-phase sytems have came to our interest to investigate the feasibility of extracting bioactive phenolics from Phanerochaete chyrsosporium biomass using this method. As far as concerned, there are no reports on the direct extraction of bioactive phenolics from this fungal biomass using aqueous two-phase system. In this project, we propose to use Phanerochaete chyrsosporium biomass because our previous result shows the biomass contained higher concentration of phenolics (506±0.09 GAE mg/l) and exhibited higher scavenging activity (84.21±0.01%) against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals compared to phenolics extracted from the fermentation broth. Effect of solvent concentration using ethyl acetate shows that the phenolics extracted with low concentration of ethyl acetate in water exhibited higher scavenging activity against DPPH and this result probably due to high water content of the extraction system which reduced the loss of biological activity of the compounds. Thus, the aqueous two-phase system seems a good alternative process for extraction of potential antioxidants from the fungus biomass. Therefore, proposed research is aimed of to investigate effect of partitioning parameters on the partition behavior and thermodynamic characteristics of transfer of phenolics in solid-liquid aqueous two phase extraction, to evaluate their antioxidant and antimicrobial activities and compared to the conventional solid-liquid extraction method and to analyze and compare bioactive phenolics constituents of solid-liquid aqueous two-phase extraction and conventional solid-liquid extraction method by high performance liquid chromatography (HPLC). In this project, effect of factors such as salt type and concentration, alcohol type and concentration, pH, temperature, solid to liquid ratio and solid particle size that on the partition behavior and thermodynamic characteristics of transfer of phenolics in solid-liquid aqueous two phase systems will be studied. The data that obtained from this proposed research can contribute to the establishment of best extraction process in the way to develop an effective, economical and environmental sustainable separation method for bioactive compounds from natural sources.
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