| ABSTRACT |
The development of lab-on-a-chip devices for biochemical analysis has seen an explosive growth over the past decade. This emerging technology has evolved into a number of applications such as clinical analysis (blood gas analysis, glucose/lactate analysis, etc.), DNA analysis (including nucleic acid sequence analysis), proteomics analysis (proteins and peptides), combinatorial synthesis/analysis, immunoassays, toxicity monitoring, and even forensic analysis applications. A significant application area for this technology is clinical diagnostics. Specifically for clinical diagnostics, diseases, including toxicity, can be diagnosed by performing various biochemicals analyzes and by observation of symptoms. The early, rapid, and sensitive detection of the disease state is a vital goal for clinical diagnoses. The biochemical changes in the patient¿s blood can signal organ damage or dysfunction prior to observable microscopic cellular damages or other symptoms. So there has been a large demand for the development of an easy-to-handle and inexpensive clinical diagnostic biochip using fully integrated plastic microfluidic chips, which has the sampling/identifying capability of fast and reliable measurements of metabolic parameters from a human body with minimum invasion. Lab-on-chip titanium dioxide nanoparticles-biosensor with integrated interdigitated electrodes for low concentration and single bio-molecule label free detection that leads to Escherichia coli (E.coli) DNA sample is developed. This biochip design we present here is, specifically optimized for research and development purpose which eventually to be commercialized. Hence, to fulfill the research, six types of gap design namely, 1, 2, 4, 7, 10, 12 µm have been proposed without changing the design concept. Therefore, it is suitable to be made as a kit with an electronic reader to show the concentration value and multiple detection results. The clear objective of the device can easily penetrate into biosensor and biomedical market for clinical usages. This huge market guarantees reasonable fortune in the future for the country.
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